Coding

Part:BBa_K2267024

Designed by: HE, BOTAO   Group: iGEM17_TUST_China   (2017-10-24)


SpyTag-GFP

SpyTag-GFP is a part containing SpyTag and green fluorescent protein fusion, which can be used to detect if the SpyTag has formed irreversible bond with SpyCatcher well after washing. Two coding sequences, Escherichia coli RBS and 6*His tag, are added in this part; thus, provides specific purification. Originated from Streptococcus pyogenes fibronectin-binding protein FbaB, the SpyTag-SpyCatcher system is commonly used for binding, labeling, immobilization and creating new kinds of application. More application can be created by replacing the GFP sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 866


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Categories
Parameters
None